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Journal of Jilin Agricultural University. 2008, (04) Core

In Vitro Callus Induction and Shoot Initiation of American Chestnut （Castanea dentata）English Full Text

YANG Guo-chen1,LU Zhong-ge(Cindy)1,Theophilus M.Asante1,Paul E.Read2(1.Department of Natural Resources and Environmental Design,North Carolina A＆T State University,Greensboro NC 27411,USA;2.Department of Agronomy and Horticulture,University of Nebraska,Lincoln NE 68583,USA)

Abstract: In vitro callus induction and shoot initiation of the American chestnut（Castanea dentata）were investigated in response to different plant growth regulators（auxins and cytokinins）in the culture medium.Micropropagated shoots were used as explant materials.Auxins included indolebutyric acid（IBA）,naphthaleneacetic acid（NAA）,2,4-dichlorophenoxyacetic acid（2,4-D）,and 2,4,5-trichlorophenoxyacetic acid（2,4,5-T）.Cytokinins included kinetin,N-（2-chloro-4-pyridyl）-N’-phenylurea（CPPU）,thidiazuron（TDZ）,and zeatin.Woody plant medium（WPM）salts containing 3% sucrose and 0.7% agar were used as the basic culture medium.Differences in the number of shoots（primodia）,morphology of micropagated shoots,and amount of callus were observed between the plant growth regulator treatments and concentrations.Explants cultured in media containing cytokinins produced more shoots（primodia）with TDZ producing the most,followed by CPPU,kinetin and zeatin.Explants cultured in media containing auxins enhanced callus induction with 2,4,5-T achieving the most,followed by 2,4-D,IBA and NAA.
Keywords：

chestnut; auxin; cytokinin; tissue culture;

  • DOI：

    10.13327/j.jjlau.2008.04.027

  • Series：

  • Subject：

  • Classification Code：

    S664.2

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