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Chinese Journal of Experimental Traditional Medical Formulae. 2015, (24) Core

Comparison of HPLC Characteristic Spectrum of Flavonoids on Leaves of Dendrobium officinale from Three Provenance SourcesChinese Full Text

HUANG Yue-chun;XIE Zhen-shan;REN Jin;YE Jia-hong;WEI Gang;The First Affiliated Hospital of Guangzhou University of Chinese Medicine;Guangzhou University of Chinese Medicine;

Abstract: Objective: To study the HPLC characteristic spectrum of flavonoids on leaves of Dendrobium officinale from different provenance sources and initially compare the characteristic spectrum among Danxia landform（ Guangdong,Fujian,Zhejiang and Jiangxi）,Tiepilan（ Guangxi,Yunnan） and native species from Zhejiang.Method: HPLC was used with Kromasil 100-5 C18 column（ 4. 6 mm × 250 mm,5 μm）; with methanol-water（ gradient elution） as a mobile phase,detection wavelength of 340 nm,column temperature of 25 ℃,and flow rate of 0. 8 m L·min-1. A common mode was generated with Traditional Chinese medicine（ TCM） chromatographic fingerprint similarity evaluation software system（ 2004 A version） by the State Pharmacopoeia Commission. Result:14 common peaks were marked in 23 batches of leaves of D. officinale. Among them,12 common peaks were marked in 10 batches of samples from Danxia landform and the similarity was 0. 880-0. 996. 13 common peaks were marked in 6 batches of samples from Zhejiang landform and the similarity was 0. 774-0. 981. 9 common peaks were marked in 7 batches of samples from Tiepilan landform（ relative peak area was larger for peak 10 and peak 11） and the similarity was 0. 922-0. 974. The similarities of Danxia landform with 6 batches from Zhejiang and 7 batches from Tiepilan were 0. 395 and 0. 061,respectively. The similarity of Tiepilan landform with 6 batches from Zhejiang was 0. 370 only. Conclusion: The method is accurate and reliable,and has a better reproducibility. The main flavonoids components of the same provenance sources are basically the same,indicating internal stability of D. officinale,but significant difference was present in components and ratio for different regions and provenance sources,providing a reference to quickly discriminate the provincial characteristics among Danxia landform,Tiepilan and native species from Zhejiang.
Keywords：

leaves of Dendrobium officinale; population district; provenance source from Danxia landform; Tiepilan; native species from Zhejiang; characteristic spectrum; HPLC;

  • DOI：

    10.13422/j.cnki.syfjx.2015240037

  • Series：

  • Subject：

  • Classification Code：

    R284.1;O657.72

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