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Mechanism and Application of Real-time Flurecenece Quantitive PCRChinese Full Text

WANG Zhi-ping1,2(1 School of Chemistry and Life Sciences,Weinan Normal University,Weinan 714000,China; 2 Key Laboratory for Eco-environment of Multi-River Wetlands in Shaanxi Province,Weinan 714000,China)

Abstract: Real-time flurecenece quantitive PCR is developed on the basis of ordinary PCR,which quantifies the template by means of standard curve.It is specific,sensitive,simple,fast and efficient.The mechanism of real-time flurecenece quantitive PCR,and systematically demonstrated its application in medical diagnosis,food testing,environmental monitoring,biological research and other fields was reviewed.
  • DOI:

    10.15924/j.cnki.1009-5128.2012.10.018

  • Series:

  • Subject:

  • Classification Code:

    R450

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