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Periodical of Ocean University of China. 2015, (09) Core

Parentage Determination of Scapharca broughtonii Based on Microsatellite AnalysisChinese Full Text

SUN Nan;LI Qi;YU Hong;KONG Ling-Feng;College of Fisheries,Ocean University of China;

Abstract: In this study,six highly polymorphic microsatellite markers（mean He= 0.78 and PIC=0.75）were utilized to determine the pedigree and the genetic variability between brood stock and offspring in a mass spawning event of ark shell,Scapharca broughtonii.Parentage identification was performed on a total of 330 individuals,including 300 offspring and 30 candidate parents.The results showed that the assignment rate was in accordance with simulation.Using 6 microsatellites reached a successful rate of over 99%.The allelic diversity of offspring populations and brood stock was similar wirth each other,but the expected heterozygosity of offspring was less than that of parents significantly（P＜0.05）.The observed heterozygosity of offspring was a little lower than that of parents,but not significant.All the 30 parents participated in the reproduction.And the existence of multiple paternity and maternity was detected in clutches and dams in these 30 parents.Microsatellite could be effective markers in parentage determination,meanwhile the gentic diversity of hatchery population of Scapharca broughtonii need to be monitored.The information obtained in this study was useful for designing suitable management guidelines of cultured stocks in future,and provided a scientific basement for tracing origins in the releasing and enhancement of Scapharca broughtonii using microsatellite as molecular markers.
Keywords：

Scapharca broughtonii; microsatellites; parentage analysis;

  • DOI：

    10.16441/j.cnki.hdxb.20140232

  • Series：

  • Subject：

  • Classification Code：

    S917.4

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