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Journal of Medical Postgraduates. 2005, (06)

Role of P38 mitogen-activated protein kinase in expressing of cyclooxygenase-2 in human umbilical vein endothelial cellsChinese Full Text

LI Yan-bo~(1), LI Wei-min~(2), HAN Jun-yong ~(3) (1.Department of Endocrinology, 2. Department of Cardiology,the First Affiliated Hospital of Haerbin Medical University, Haerbin 150001,Heilongjiang, China; 3. Department of Cardiology, the Fifth Affiliated Hospital of Zhongshan Medical University, Zhuhai 519000, Guangdong, China)

Abstract: Objective: To investigate the role of P38 mitogen-activated protein kinase （P38MAPK） in expressing cyclooxygenase-2 （COX-2） in human umbilical vein endothelial cells （HUVEC）, in order to study the role of P38MAPK on diabetic atherosclerosis. Methods: HUVEC are separately stimulated with high glucose, advanced glycosylation end products （AGE）, high insulin and H2O2, the expression of phospho-P38MAPK and COX-2 is detected; Simultaneously, HUVEC pre-treated with SB203580 （P38MAPK special inhibitor） are stimulated with above stimulating factors, the expression of COX-2 is detected. Results: High glucose, AGE, high insulin and H2O2 can activate P38MAPK and increase the expression both of phospho-P38MAPK and COX-2 significantly; But the expression of COX-2 is inhibited by SB203580. Conclusion: P38MAPK regulates the expression of COX-2; P38MAPK is a upstream signal molecule of COX-2 and P38MAPK may be one of initial signals on atherosclerosis occurrence.
Keywords：

P38 mitogen-activated protein kinase; Cyclooxygenase-2; Diabetes; Atherosclerosis;

  • DOI：

    10.16571/j.cnki.1008-8199.2005.06.003

  • Series：

  • Subject：

  • Classification Code：

    R587.2

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