Intervention effects of Zuoguiwan containing serum on osteoblast through ERK1/2 and Wnt/β-catenin signaling pathway in models with kidney-Yang-deficiency,kidney-Yin-deficiency osteoporosis syndromesChinese Full TextEnglish Full Text (MT)
ZHANG Jian-hua;XING Jing;FAN Lian-xia;YIN Hua;The First Affiliated Hospital,Zhejiang University of Chinese Medical;Standardization Laboratory in Pharmacy College,Zhejiang University of Chinese Medicine;
Abstract: To clarify the effects of Zuoguiwan containing serum on osteoblast proliferation and alkaline phosphatase（ ALP） expression and its effects on the expression of β-catenin,ERK1,ERK2 mRNA and protein of osteoblast through ERK1/2,Wnt/β-catenin signaling pathway in models with osteoporosis（ OP） kidney-Yang-deficiency,osteoporosis（ OP） kidney-Yin-deficiency syndrome. Rat osteoporosis models were established by ovariectomy surgery,and 10 weeks after surgery,hydrocortisone was injected and thyroxine was administered by intragastric administration to establish OP kidney-Yang-deficiency rat model,and OP kidney-Yin-deficiency rat model.Osteoblasts were obtained from 24 h newborn rat skull and were identified by alkaline phosphatase and alizarin red staining. Zuoguiwan containing serum of OP,OP kidney-Yang-deficiency,and OP kidney-Yin-deficiency,as well as the blank serum were used to intervene the osteoblast,and the cells proliferation was detected by MTS. ELISA assay was used to detect ALP expression. RT-PCR assay was used to detect the mRNA expression of ERK1,ERK2,β-catenin and protein expression levels were detected by Western blot. The results showed that Zuoguiwan containing serum in OP kidney-Yin-deficiency model had stronger effect than OP kidney-Yang-deficiency in promoting osteoblast proliferation,ALP expression,osteoblast ERK1/2,Wnt/β-catenin signaling pathway related factors β-catenin,ERK1,ERK2 mRNA and protein expression levels. This was consistent with the TCM theory of " Zuoguiwan nourishes kidney Yin",providing a scientific basis for the clinical and dialectical treatment of osteoporosis. Zuoguiwan could regulate the proliferation and differentiation of bone cells by ERK1/2 and Wnt/β-catenin signaling pathway,which may be one of the mechanisms of Zuoguiwan for the prevention of osteoporosis.
Keywords：
Zuoguiwan; osteoblasts; drug containing serum; OP kidney-Yang-deficiency; OP kidney-Yin-deficiency; Wnt/β-catenin signaling pathway; ERK1/2 signaling pathway;
- DOI：
10.19540/j.cnki.cjcmm.20170907.002
- Series：
- Subject：
- Classification Code：
R285
- Mobile Reading
Read on your phone instantly
Step 1Scan QR Codes
"Mobile CNKI-CNKI Express" App
Step 2Open“CNKI Express”
and click the scan icon in the upper left corner of the homepage.
Step 3
Scan QR Codes
Read this article on your phone.
- Download
- Online Reading
Download the mobile appuse the app to scan this coderead the article.
Tips: Please download CAJViewer to view CAJ format full text.
Download: 1121 Page: 3983-3989 Pagecount: 7 Size: 449K
Citation Network
Related Literature
- Similar Article
- Reader Recommendation
- Associated Author