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China Journal of Chinese Materia Medica. 2018, (17) Core

Simultaneous determination of nine constituents of Xuebijing Injection in rat plasma and their pharmacokinetics by LC-MS/MSChinese Full Text

OUYANG Hui-zi;HE Jun;the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine;Tianjin University of Traditional Chinese Medicine;Tianjin State Key Laboratory of Modern Chinese Medicine,Co-construction of Province and Ministry,State Key Laboratory Breeding Base;

Abstract: A high performance liquid chromatography-tandem mass spectrometry（ HPLC-MS/MS） method was established for the determination of nine active ingredients of Xuebijing Injection in rat plasma and the pharmacokinetic properties of Xuebijing Injection in rats were studied after intravenous admin as the mobile phase for gradient elution. A mass spectrometry was applied with electrospray ionization（ ESI） ion source in the negative ion multiple reaction monitoring（ MRM） mode. All pharmacokinetic parameters were processed by non-compartmental analysis with DAS 2. 0 software. After intravenous administration of 6 m L·kg-1 Xuebijing Injection,the main pharmacokinetic parameters of ferulic acid,benzoylpaeoniflorin,danshensu,chlorogenic acid,rosmarinic acid,hydroxy-methyl safflower yellow A,paeoniflorin,albiflorin,and oxypaeoniflorin were as follows: Cmax（ 85. 01 ± 22. 33）,（ 56. 78 ± 22. 78）,（ 36. 05 ±8. 77）,（ 73. 73 ± 20. 26）,（ 173. 19 ± 67. 12）,（ 20 646. 96 ± 2 666. 65）,（ 21 416. 61 ± 6 427. 14）,（ 181. 88 ± 38. 38）,（ 770. 73 ± 230. 87） μg·L-1; AUC（ 0-t n）（ 801. 9 ± 112. 3）,（ 1 005. 6 ± 406. 0）,（ 714. 9 ± 237. 7）,（ 1 496. 3 ± 413. 9）,（ 2 316. 1 ± 372. 2）,（ 1 207 295. 3 ± 248 582. 6）,（ 788 781. 0 ± 308 551. 0）,（ 4 785. 4 ± 1 076. 0）,（ 33 456. 2 ± 11 946. 1） min·μg·L-1. This method is sensitive and accurate,applicable for the pharmacokinetic study of Xuebijing Injection,and provides a basis for clinical research of Xuebijing Injection.
Keywords：

Xuebijing Injection; LC-MS/MS; pharmacokinetics;

  • DOI：

    10.19540/j.cnki.cjcmm.20180611.010

  • Series：

  • Subject：

  • Classification Code：

    R285.5

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